An alternative name for the technique is Cleaved Amplified Polymorphic Sequence (CAPS) assay. Therefore, more samples can be analyzed in a shorter time. However, PCR can be used to amplify very small amounts of DNA, usually in 2-3 hours, to the levels required for RFLP analysis. Isolation of sufficient DNA for RFLP analysis is time consuming and labor intensive. Typically, in species with moderate to high polymorphism rates, two to four restriction endonucleases are used such as EcoRI
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